Chloroquine dna intercalation

Discussion in 'Northwest Pharmacy Canada' started by elgato, 01-Mar-2020.

  1. mcshel New Member

    Chloroquine dna intercalation


    Note: based on a RGCC chemosensitivity analysis I have seen at a German clinic, Hydroxychloroquine has been effective in killing the cancer cells of 5 out 7 patients that were tested. It is one of very few available drugs that inhibits autophagy, a mechanism associated with its anticancer properties.

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    Chloroquine is a member of quinolone family and is a weak intercalating agent. Chloroquine is used for treating amebiasis, rheumatoid arthritis, discoid and systemic lupus erythematosus. Application DNA intercalator. Also used to increase transfection efficiency. Chloroquine diphosphate salt has been used • in in vitro antiplasmodial assays Unwinding of the double strands of the DNA helix is a hallmark feature of intercalating drugs, such as chloroquine, ethidium bromide and m-AMSA. As part of characterization of novel agents, it is often important to know whether a novel compound is a DNA intercalator. The results obtained suggest that even gentle protein denaturation is sufficient to reduce considerably the effectiveness of the DNA loop migration due to an increase in the loops size. The same effect was observed under local DNA unwinding upon chloroquine intercalation around the sites of the attachment of DNA to proteins.

    In cancer, authophagy is the process used by cancer cells to “self-eat” in order to survive. Specifically, if authophagy is prolonged this will become a lethal process to cancer. duing chemotheraphy, radiotheraphy, etc.) authophagy is used by cancer cells to survive. However, note that Chloroquine has other properties as well that may be very well related to anti cancer mechanism, such as zinc ionophore, and others (see below the section on mechanisms).

    Chloroquine dna intercalation

    Influence of chloroquine on kinetics of single-cell gel. - CORE, DNA Intercalator/Unwinding Kit, pHOT-1 DNA, mAMSA, DNA.

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  6. Chloroquine has been shown to inhibit RNA and DNA syn- 18-20 thesis in prokaryotes and in Plas- 2, modium knowlesi. Chloroquine, like other intercalators, may work by altering three-dimensional structure. For exam- ple, there is evidence that DNA may form left-handed helices Z-DNAin transcriptionally active regions.

    • Chloroquine as intercalator a hypothesis revived..
    • Protein intercalation in DNA as one of main modes of fixation of the..
    • Chloroquine - FDA prescribing information, side effects and uses.

    The response of ssDNA sensor was caused by interaction of phosphates groups of DNA with chloroquine cations. However, the response of dsDNA sensor was caused not only by interaction of phosphates groups of DNA with chloroquine cations but also by intercalation of chloroquine cations into dsDNA double strand helix, which leads the response greater. In biochemistry, intercalation is the insertion of molecules between the bases of DNA. This process is used as a method for analyzing DNA and it is also the basis of certain kinds of poisoning. Clathrates are chemical substances consisting of a lattice that traps or contains molecules. Usually, clathrate compounds are polymeric and completely envelop the guest molecule. Chloroquine is reported in the literature to be a weak genotoxic agent that may elicit both gene mutations and chromosomal/DNA breaks. Mechanisms may involve DNA intercalation or induction of oxidative stress. Both positive and negative results have been reported with in vitro reverse gene mutation assays and with in vivo animal studies.

     
  7. Genna User

    Joint damage worries and aching hands are no fun, and if you’re experiencing those kinds of problems because of rheumatoid arthritis you may have options that can help you feel better. Methotrexate for RA Side Effects, Effectiveness Does anyone take methotrexate and hydroxychloroquine. Rheumatoid Arthritis Treatment Options Johns Hopkins.
     
  8. SLrat New Member

    Joint damage worries and aching hands are no fun, and if you’re experiencing those kinds of problems because of rheumatoid arthritis you may have options that can help you feel better. Long-Term Side Effects of Plaquenil for Rheumatoid Arthritis Healthfully Treatments for Rheumatoid Arthritis Taking Plaquenil for Rheumatoid Arthritis
     
  9. errordnk XenForo Moderator

    The lysosomal inhibitor, chloroquine, increases cell surface. Sep 15, 2013 Chloroquine is a lysosomotropic agent as it is usually prepared as a diprotic weak base pKa 8.5. The unprotonated form of chloroquine preferentially accumulates in lysosomes as it rapidly diffuses across cell/organelle membranes.

    Inhibition of autophagy with bafilomycin and chloroquine.