Chloroquine resazurin assay results

Discussion in 'Canadian Pharmaceuticals Online' started by Jim Beam, 13-Mar-2020.

  1. AnPetrushin XenForo Moderator

    Chloroquine resazurin assay results


    Current treatment options are, at best, palliative. The objectives of this study were to evaluate a panel of nineteen candidate compounds for antiviral activity against FCo V to determine viable candidates for therapy.

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    A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. Search results for Resazurin at Sigma-Aldrich. Compare Products Select up to 4 products. *Please select more than one item to compare Resazurin Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. This kit performs at least as well as other commercial resazurin-based cell proliferation assay kits such as alamarBlue® or CellTiter-Blue®.

    Plaque reduction and virus yield reduction assays were performed to confirm antiviral effects of candidate compounds identified during screening, and the possible antiviral mechanisms of action of these compounds were investigated using virucidal suspension assays and CPE inhibition and IFA-based time of addition assays. A resazurin-based cytopathic effect inhibition assay, which detects viable cells through their reduction of the substrate resazurin to fluorescent resorufin, was developed for screening compounds for antiviral efficacy against FCo V.

    Chloroquine resazurin assay results

    Establishment and application of a novel isothermal., Resazurin Sigma-Aldrich

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  7. Thaw out Resazurin solution if kept frozen and warm it to 37°C to ensure all components are completely in solution. Plate cells in 96-well plate black plate with clear bottom. Typical seed cell number is 0.02–2 x 10 5 cells/well depending on cell growth rate.

    • Resazurin Cell Viability Kit Protocol CST.
    • Resazurin Cell Viability Assay Kit Biotium.
    • Resazurin Products R&D Systems.

    The resazurin test may be a valuable time saving tool if properly conducted and intelligently interpreted, but should be supplemented by microscopic examination. Results on the reliability of the resazurin tests are conflicting. One study in comparing the resazurin test with the Breed microscopic method on 235 samples found the test reliable. Get high quality cell viability results and instant time savings with PrestoBlue® Cell Viability Reagent. PrestoBlue® is a ready to use cell permeable resazurin-based solution that functions as a cell viability indicator by using the reducing power of living cells to quantitatively measure the proliferation of cells. The resazurin dye can be measured in fluorescence or absorbance mode. However fluorescence mode measurement offers greater assay linearity, reproducibility, robustness and sensitivity. Resazurin assay protocol summary - add resazurin reagent to cells in growth media and incubate for 4 hr at 37ºC - analyze with microplate reader

     
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